Most cells are transparent—in other words, they are not very good at reflecting or absorbing light. To look at them under a microscope thus requires trickery. Many of these tricks kill the cells, and even those that keep them alive look only at slices through each cell, rather than seeing the whole thing in three dimensions.
Michael Feld, of the Massachusetts Institute of Technology, and his colleagues, think they can change that. They have invented a way to look at cells that are still alive. Moreover, they can do so in three dimensions. Their method is called tomographic phase microscopy, and it is reported in this week's Nature Methods. Instead of relying on absorbed or reflected light, Dr. Feld's technique celebrates transparency by looking at light that gets through unaltered. It does so by measuring a property called the refractive index.
This index measures the speed of light in a material. (Light zips along at the actual “speed of light”, faster than which nothing can go, only when it is travelling through a vacuum.) The different components of a cell, though transparent, have different refractive indices. Dr. Feld and his team therefore set out to map what these differences are, with a view to using them to distinguish between cellular components.
To measure the refractive indices of different parts of a cell they use a technique called interferometry, which involves splitting a beam of light in two. One half, known as the object beam, passes through the cell; the other is directed along a different path and acts as a reference. The length of the reference path is such that if no sample is present, the two daughter beams will be as perfectly in phase when they meet as they were when they were separated. The crests and the troughs of their waves will reinforce each other, and the result will be brightness. The more that the light passing through the sample is slowed down, however, the more the two beams will be out of phase. Crest will fall on trough, and the result will be darkness. It is this phase shift that gives Dr. Feld's new form of microscopy its name.
A single pair of beams does not, however, produce a useful image. To do that requires scanning the object beam through the target about a hundred different ways. From the refractive index of each path it is possible—with the application of some suitably crunchy computing power—to produce a three-dimensional image.
To test his idea, Dr. Feld looked at cervical-cancer cells. If you identify this cancer early, the patient will probably survive. Miss it, and she will die. Dr. Feld wondered if the changes that occur during cancer would show up using his new method. They did, in a part of the cell called the nucleolus. This is the place where the components of protein factories are made. Since cancer cells grow rapidly, and thus have a high demand for proteins, it was a likely place to expect changes.
Dr. Feld also has plans to use beams of different colours, since each colour has a slightly different refractive index in a given material. That would provide extra data for the computer to chew on, and probably result in better pictures. With enough pictures, Dr. Feld's technique may make biology as transparent as the cells it studies.
1. Tomographic phase microscopy is different from the other tricks that look at cells in that _____.
[A] the light that gets through the cells is unaltered in tomographic phase microscopy
[B] it does not require the trickery to kill cells
[C] it is not dependent on absorbed or reflected light
[D] it could see all the components of the cells from three dimensions
2. Which one of the following statements is TRUE of the speed of light in materials?
[A] Light runs the fastest when it passes through transparent materials in the real world.
[B] The speed of light can only be precisely measured when the light is traveling in vacuum.
[C] The speed of light in any material is slower than the actual “speed of light”.
[D] The speed of light shifts when the light travels through different cells.
3. The result of darkness in the technique of interferometry implies that _____.
[A] there is no light passing through the sample
[B] the refractive index of the sample is very great
[C] the speed of the light passing through the sample is very low
[D] the two daughter beams weaken each other
4. Dr. Feld's method could be applied into identifying cancer early by _____.
[A] detecting changes of refractive indices in the components of protein factories
[B] finding out the changes of the refractive indices in some parts of the cervical-cancer cells
[C] examining the changes of the refractive indices in the nucleolus
[D] identifying the changes of the refractive indices in part of the nucleolus of the cancer cells
5. The best title of the passage could be _____.
[A] Image of the Transparent Cells
[B] New Technique of Celebrating Transparency
[C] Refractive Indices of the Transparent Cells
[D] New Method of Detecting Cancer Cells
1. Tomographic phase microscopy is different from the other tricks that look at cells in that _____.
[A] the light that gets through the cells is unaltered in tomographic phase microscopy
[B] it does not require the trickery to kill cells
[C] it is not dependent on absorbed or reflected light
[D] it could see all the components of the cells from three dimensions
1. X線(xiàn)斷層階段顯微法和其他觀察細(xì)胞的方法的不同之處在于 _____。
[A] 穿過(guò)細(xì)胞的光在X線(xiàn)斷層階段顯微法中是沒(méi)有發(fā)生改變的
[B] 它不要求殺死細(xì)胞
[C] 它不依靠被吸收或反射的光
[D] 它可以從三個(gè)方位看到細(xì)胞的所有組成成分
答案:C 難度系數(shù):☆☆☆
分析:細(xì)節(jié)題。選項(xiàng)A,第二段提到的是一些光穿過(guò)細(xì)胞時(shí)沒(méi)有改變,但不代表所有的都沒(méi)改變。選項(xiàng)B,根據(jù)第一段和第二段,該方法可以觀察活細(xì)胞,但是之前的一些方法也可以觀察活細(xì)胞,因此并不是二者的不同之處。選項(xiàng)C,第二段提到,該方法沒(méi)有依靠被吸收或反射的光。選項(xiàng)D的內(nèi)容文章中沒(méi)有提到。因此,答案為C。
2. Which one of the following statements is TRUE of the speed of light in materials?
[A] Light runs the fastest when it passes through transparent materials in the real world.
[B] The speed of light can only be precisely measured when the light is traveling in vacuum.
[C] The speed of light in any material is slower than the actual “speed of light”.
[D] The speed of light shifts when the light travels through different cells.
2. 關(guān)于光在物質(zhì)中的傳播速度,下列哪個(gè)陳述是正確的?
[A] 光在穿過(guò)現(xiàn)實(shí)世界中的透明物體時(shí)的速度是最快的。
[B] 光速只有在真空狀態(tài)下才能夠被準(zhǔn)確測(cè)量。
[C] 在任何物質(zhì)中,光的速度都比“真正的光速”慢。
[D] 光穿過(guò)不同的細(xì)胞時(shí),速度也會(huì)發(fā)生變化。
答案:C 難度系數(shù):☆☆☆
分析:細(xì)節(jié)題。根據(jù)第三段,光在真空中速度最快,而真實(shí)世界中也存在真空,因此選項(xiàng)A是錯(cuò)誤的。選項(xiàng)B的錯(cuò)誤則在于,雖然其表述正確,但是卻與題干要求討論的“光在物質(zhì)中的傳播速度”無(wú)關(guān)。選項(xiàng)C,文章也提到了這點(diǎn),沒(méi)有別的物體的運(yùn)行速度比光速快。選項(xiàng)D,光通過(guò)不同的細(xì)胞時(shí),折射率不同,而與速度無(wú)關(guān)。因此,答案為C。
3. The result of darkness in the technique of interferometry implies that _____.
[A] there is no light passing through the sample
[B] the refractive index of the sample is very great
[C] the speed of the light passing through the sample is very low
[D] the two daughter beams weaken each other
3. 在干涉測(cè)量法中,黑暗的結(jié)果表示 _____。
[A] 沒(méi)有光通過(guò)樣本
[B] 樣本的折射率很大
[C] 通過(guò)樣本的光速很慢
[D] 兩個(gè)子光束彼此削弱
答案:D 難度系數(shù):☆
分析:推理題。根據(jù)第四段,當(dāng)通過(guò)物體的光的速度減慢得越多,和另一束光束的異相程度就越大,波峰和波谷相互抵消,因此是黑暗的。因此,選項(xiàng)D最為符合。
4. Dr. Feld's method could be applied into identifying cancer early by _____.
[A] detecting changes of refractive indices in the components of protein factories
[B] finding out the changes of the refractive indices in some parts of the cervical-cancer cells
[C] examining the changes of the refractive indices in the nucleolus
[D] identifying the changes of the refractive indices in part of the nucleolus of the cancer cells
4. Feld博士的方法可以運(yùn)用到早期發(fā)現(xiàn)癌癥上,這要通過(guò) _____來(lái)實(shí)現(xiàn)。
[A] 探測(cè)蛋白質(zhì)工廠組成部分折射率的變化
[B] 發(fā)現(xiàn)部分子宮癌細(xì)胞中折射率的變化
[C] 探測(cè)細(xì)胞核中折射率的變化
[D] 找出部分癌細(xì)胞的細(xì)胞核中折射率的變化
答案:C 難度系數(shù):☆☆
分析:根據(jù)第六段:They did, in a part of the cell called the nucleolus. This is the place where the components of protein factories are made. Since cancer cells grow rapidly, and thus have a high demand for proteins, it was a likely place to expect changes. 可知,F(xiàn)eld博士是在細(xì)胞核中發(fā)現(xiàn)了這種變化,因?yàn)榘┘?xì)胞的生長(zhǎng)需要大量的蛋白質(zhì),而細(xì)胞核是生產(chǎn)蛋白質(zhì)的地方,因此,如果有變化就應(yīng)該可以體現(xiàn)在細(xì)胞核中。所以,答案為C。
5. The best title of the passage could be _____.
[A] Image of the Transparent Cells
[B] New Technique of Celebrating Transparency
[C] Refractive Indices of the Transparent Cells
[D] New Method of Detecting Cancer Cells
5. 這篇文章最好的題目是 _____。
[A] 透明細(xì)胞的圖像
[B] 研究透明的新技術(shù)
[C] 透明細(xì)胞的折射率
[D] 發(fā)現(xiàn)癌細(xì)胞的新方法
答案:B 難度系數(shù):☆☆☆
分析:主旨題。這篇文章主要講述了研究細(xì)胞折射率的新方法,通過(guò)這種新方法可以做許多研究,如細(xì)胞的樣子和癌細(xì)胞的早期發(fā)現(xiàn)。因此,答案為B。
大多數(shù)的細(xì)胞都是透明的,也就是說(shuō),它們沒(méi)有很強(qiáng)的反射或吸收光的能力。因此,要在顯微鏡下看到它們就需要一定的技巧了。許多技巧都會(huì)殺死細(xì)胞,而那些能保證它們存活的技巧只能使人們看到各個(gè)細(xì)胞的切片,而不是從三維立體的角度來(lái)觀察細(xì)胞。
麻省理工大學(xué)的Michael Feld及其同事認(rèn)為他們可以改變這一點(diǎn)。他們發(fā)明了觀察活細(xì)胞的方法,而且還可以觀察到立體的細(xì)胞。他們的方法叫作X線(xiàn)斷層階段顯微法,并刊登在本周的《自然方法》上。Feld博士不是通過(guò)那些被吸收或反射的光,而是利用了細(xì)胞透明特性,觀察光穿過(guò)細(xì)胞后是否發(fā)生了改變。該方法是通過(guò)測(cè)量光的折射率來(lái)實(shí)現(xiàn)的。
折射率測(cè)量光在某種物質(zhì)中的傳播速度。(光只有在真空中時(shí)才以真正的“光速”來(lái)傳播,比其他任何物體的速度都要快。)雖然細(xì)胞的不同成分是透明的,卻有不同的折射率。Feld博士及其研究小組因此開(kāi)始尋找這些不同,希望可以通過(guò)不同的折射率來(lái)辨別細(xì)胞的各種成分。
為了估測(cè)某一細(xì)胞不同組成部分的折射率,他們使用了一種叫干涉測(cè)量的方法,將一束光線(xiàn)一分為二,其中一束叫作物體光束,能穿過(guò)細(xì)胞;另外一束則沿著不同的路線(xiàn)前進(jìn),作為參照。參照路線(xiàn)的長(zhǎng)度一定,如果沒(méi)有物體,那么這兩個(gè)光束就會(huì)同相,與它們分開(kāi)時(shí)的情況一樣。兩束光的波峰和波谷會(huì)互相加強(qiáng),結(jié)果就是形成了非常明亮的光。通過(guò)物體的光的速度減慢得越多,兩個(gè)光束異相的程度就越大。此時(shí),波峰會(huì)與波谷重合,最終結(jié)果就是黑暗。正是因?yàn)橄嗟淖兓?,F(xiàn)eld博士的新顯微形式才有了自己的名稱(chēng)。
但是一對(duì)光束不會(huì)制造出有用的圖像來(lái)。圖像的生成需要將通過(guò)物體的光束以100種不同的方式來(lái)進(jìn)行掃描。從每條路徑的折射率,再運(yùn)用一些適當(dāng)?shù)挠?jì)算,就有可能可以生成三維圖像。
為了驗(yàn)證他的想法,F(xiàn)eld博士研究了子宮癌細(xì)胞。越早辨認(rèn)出該細(xì)胞,病人存活的幾率就越大。如果忽略了該細(xì)胞,病人就會(huì)死亡。Feld博士想知道,使用自己的新方法是否能發(fā)現(xiàn)癌癥期間的一些變化。他們?cè)诩?xì)胞核中發(fā)現(xiàn)了這種變化。細(xì)胞核是生產(chǎn)構(gòu)成蛋白質(zhì)的成分的地方。因?yàn)榘┘?xì)胞的生長(zhǎng)速度極快,因此需要大量的蛋白質(zhì),所以這里是最有可能發(fā)生改變的地方。
Feld博士還計(jì)劃使用不同顏色的光束,因?yàn)槊糠N顏色在某一物質(zhì)中的折射率都略有不同。這就為計(jì)算機(jī)成像提供了更多的數(shù)據(jù),而且可能會(huì)產(chǎn)生更好的圖像。有了足夠多的圖像,F(xiàn)eld博士的技術(shù)就可以讓生物學(xué)變得透明起來(lái),就像他研究的那些透明細(xì)胞一樣。